nucleocapsid n gene Search Results


nucleocapsid (n) gene sensitivity  (Liferiver Bio Tech Corp)
90
Liferiver Bio Tech Corp nucleocapsid (n) gene sensitivity
Nucleocapsid (N) Gene Sensitivity, supplied by Liferiver Bio Tech Corp, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nucleocapsid (n) gene sensitivity/product/Liferiver Bio Tech Corp
Average 90 stars, based on 1 article reviews
nucleocapsid (n) gene sensitivity - by Bioz Stars, 2026-05
90/100 stars
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expression plasmids encoding nucleocapsid (n) gene rsv or hmpv  (Biomatik)
90
Biomatik expression plasmids encoding nucleocapsid (n) gene rsv or hmpv
HMPV infection is elevated in NECs from asthmatics with no associated defect in IFN production. a <t>RSV</t> or hMPV N gene copy number was quantified by RT-qPCR using total RNA extracted from NECs 24 h p.i. (MOI 3) and <t>control</t> <t>plasmids</t> for RSV and hMPV N gene and β-actin gene expression. b Secreted IFN-β was quantified by ALPHAlisa. c Secreted IFN-λ1 was quantified by ELISA. n = 10 for both asthmatics and non-asthmatics. The bottom and top of the box plots represent the 5th and 95th percentiles, the bar represents the median and the whiskers represent minimum and maximum. A GEE approach was used to compare groups and the interaction between disease status and virus was significant for the viral load but not for the IFN response. * p < 0.05, ** p < 0.01 and **** p < 0.0001 show statistical significance.
Expression Plasmids Encoding Nucleocapsid (N) Gene Rsv Or Hmpv, supplied by Biomatik, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/expression plasmids encoding nucleocapsid (n) gene rsv or hmpv/product/Biomatik
Average 90 stars, based on 1 article reviews
expression plasmids encoding nucleocapsid (n) gene rsv or hmpv - by Bioz Stars, 2026-05
90/100 stars
  Buy from Supplier
nucleocapsid n gene  (Bioedit Company)
86
Bioedit Company nucleocapsid n gene
HMPV infection is elevated in NECs from asthmatics with no associated defect in IFN production. a <t>RSV</t> or hMPV N gene copy number was quantified by RT-qPCR using total RNA extracted from NECs 24 h p.i. (MOI 3) and <t>control</t> <t>plasmids</t> for RSV and hMPV N gene and β-actin gene expression. b Secreted IFN-β was quantified by ALPHAlisa. c Secreted IFN-λ1 was quantified by ELISA. n = 10 for both asthmatics and non-asthmatics. The bottom and top of the box plots represent the 5th and 95th percentiles, the bar represents the median and the whiskers represent minimum and maximum. A GEE approach was used to compare groups and the interaction between disease status and virus was significant for the viral load but not for the IFN response. * p < 0.05, ** p < 0.01 and **** p < 0.0001 show statistical significance.
Nucleocapsid N Gene, supplied by Bioedit Company, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nucleocapsid n gene/product/Bioedit Company
Average 86 stars, based on 1 article reviews
nucleocapsid n gene - by Bioz Stars, 2026-05
86/100 stars
  Buy from Supplier

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HMPV infection is elevated in NECs from asthmatics with no associated defect in IFN production. a RSV or hMPV N gene copy number was quantified by RT-qPCR using total RNA extracted from NECs 24 h p.i. (MOI 3) and control plasmids for RSV and hMPV N gene and β-actin gene expression. b Secreted IFN-β was quantified by ALPHAlisa. c Secreted IFN-λ1 was quantified by ELISA. n = 10 for both asthmatics and non-asthmatics. The bottom and top of the box plots represent the 5th and 95th percentiles, the bar represents the median and the whiskers represent minimum and maximum. A GEE approach was used to compare groups and the interaction between disease status and virus was significant for the viral load but not for the IFN response. * p < 0.05, ** p < 0.01 and **** p < 0.0001 show statistical significance.

Journal: Journal of Innate Immunity

Article Title: Human Metapneumovirus Impairs Apoptosis of Nasal Epithelial Cells in Asthma via HSP70

doi: 10.1159/000449101

Figure Lengend Snippet: HMPV infection is elevated in NECs from asthmatics with no associated defect in IFN production. a RSV or hMPV N gene copy number was quantified by RT-qPCR using total RNA extracted from NECs 24 h p.i. (MOI 3) and control plasmids for RSV and hMPV N gene and β-actin gene expression. b Secreted IFN-β was quantified by ALPHAlisa. c Secreted IFN-λ1 was quantified by ELISA. n = 10 for both asthmatics and non-asthmatics. The bottom and top of the box plots represent the 5th and 95th percentiles, the bar represents the median and the whiskers represent minimum and maximum. A GEE approach was used to compare groups and the interaction between disease status and virus was significant for the viral load but not for the IFN response. * p < 0.05, ** p < 0.01 and **** p < 0.0001 show statistical significance.

Article Snippet: RSV and hMPV intracellular RNA was quantified using SYBR green dye (QIAGEN), specific primers (online suppl. table S1 ; for all online suppl. material, see www.karger.com/doi/10.1159/000449101 ) and standard curves were generated using 10-fold serial dilutions of expression plasmids encoding the nucleocapsid (N) gene of RSV or hMPV (Biomatik).

Techniques: Infection, Quantitative RT-PCR, Expressing, Enzyme-linked Immunosorbent Assay

Apoptosis in response to hMPV is reduced in NECs from asthmatics. a Representative Annexin-V/7-AAD staining of NECs infected with hMPV or RSV at a MOI of 3 at 24 h p.i. b Quantification of the proportion of Annexin-V+ NECs at 24 h p.i. when normalized with Annexin-V-positive uninfected cells. n = 5 for both asthmatics and non-asthmatics. The bottom and top of the box plots represent the 5th and 95th percentiles, the bar represents the median and the whiskers represent minimum and maximum. A GEE approach was used to compare groups and the interaction between disease status and virus was significant. Statistical significance between asthmatic and non-asthmatic NECs is indicated by ** p < 0.01 and **** p < 0.0001.

Journal: Journal of Innate Immunity

Article Title: Human Metapneumovirus Impairs Apoptosis of Nasal Epithelial Cells in Asthma via HSP70

doi: 10.1159/000449101

Figure Lengend Snippet: Apoptosis in response to hMPV is reduced in NECs from asthmatics. a Representative Annexin-V/7-AAD staining of NECs infected with hMPV or RSV at a MOI of 3 at 24 h p.i. b Quantification of the proportion of Annexin-V+ NECs at 24 h p.i. when normalized with Annexin-V-positive uninfected cells. n = 5 for both asthmatics and non-asthmatics. The bottom and top of the box plots represent the 5th and 95th percentiles, the bar represents the median and the whiskers represent minimum and maximum. A GEE approach was used to compare groups and the interaction between disease status and virus was significant. Statistical significance between asthmatic and non-asthmatic NECs is indicated by ** p < 0.01 and **** p < 0.0001.

Article Snippet: RSV and hMPV intracellular RNA was quantified using SYBR green dye (QIAGEN), specific primers (online suppl. table S1 ; for all online suppl. material, see www.karger.com/doi/10.1159/000449101 ) and standard curves were generated using 10-fold serial dilutions of expression plasmids encoding the nucleocapsid (N) gene of RSV or hMPV (Biomatik).

Techniques: Staining, Infection

Caspase-3/7 and caspase-9 activity in NECs from asthmatic subjects is reduced in response to hMPV. Caspase-3/7 (a, b) and caspase-9 (c, d) activation was quantified by luminescence detection at the indicated time points within 24 h following hMPV or RSV infection (MOI 3). n = 5 for both asthmatics and non-asthmatics; median ± range. Caspase-3/7 (e) and caspase-9 (f)activation were quantified at 4, 8 and 12 h after treatment with 10 µM AT-101. n = 5 for both asthmatics and non-asthmatics; median ± range. A GEE approach was used to compare groups and the interaction between disease status and time following hMPV infection was significant. Statistical significance between asthmatic and non-asthmatic NECs is indicated by ** p < 0.01 and **** p < 0.0001. Statistical significance between infected (or treated) and uninfected (or untreated) NECs is indicated by † p < 0.05, †† p < 0.01, ††† p < 0.001 and †††† p < 0.0001.

Journal: Journal of Innate Immunity

Article Title: Human Metapneumovirus Impairs Apoptosis of Nasal Epithelial Cells in Asthma via HSP70

doi: 10.1159/000449101

Figure Lengend Snippet: Caspase-3/7 and caspase-9 activity in NECs from asthmatic subjects is reduced in response to hMPV. Caspase-3/7 (a, b) and caspase-9 (c, d) activation was quantified by luminescence detection at the indicated time points within 24 h following hMPV or RSV infection (MOI 3). n = 5 for both asthmatics and non-asthmatics; median ± range. Caspase-3/7 (e) and caspase-9 (f)activation were quantified at 4, 8 and 12 h after treatment with 10 µM AT-101. n = 5 for both asthmatics and non-asthmatics; median ± range. A GEE approach was used to compare groups and the interaction between disease status and time following hMPV infection was significant. Statistical significance between asthmatic and non-asthmatic NECs is indicated by ** p < 0.01 and **** p < 0.0001. Statistical significance between infected (or treated) and uninfected (or untreated) NECs is indicated by † p < 0.05, †† p < 0.01, ††† p < 0.001 and †††† p < 0.0001.

Article Snippet: RSV and hMPV intracellular RNA was quantified using SYBR green dye (QIAGEN), specific primers (online suppl. table S1 ; for all online suppl. material, see www.karger.com/doi/10.1159/000449101 ) and standard curves were generated using 10-fold serial dilutions of expression plasmids encoding the nucleocapsid (N) gene of RSV or hMPV (Biomatik).

Techniques: Activity Assay, Activation Assay, Infection

HMPV induced the expression of ER stress and unfolded protein response markers in NECs from both asthmatic and non-asthmatic subjects. Grp78 (a), sXBP1 (b), CHOP (c), GADD34 (d), PUMA (e) and NOXA (f) mRNA expression in NECs at 8, 12 and 24 h following infection with hMPV or RSV (Grp78 and sXBP1 only; MOI of 3). RT-qPCR was performed using total cellular RNA. Fold induction was calculated using 2−ΔΔCt normalized to the expression of β-actin gene. n = 5 for both asthmatic and non-asthmatic subjects. The bottom and top of the box plots represent the 5th and 95th percentiles, the bar represents the median and the whiskers represent minimum and maximum. A GEE approach was used to compare groups and the interaction between disease status and time (hMPV time course) or between disease status and virus (hMPV vs. RSV at 24 h p.i.) was not significant. * p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001 indicate statistical significance.

Journal: Journal of Innate Immunity

Article Title: Human Metapneumovirus Impairs Apoptosis of Nasal Epithelial Cells in Asthma via HSP70

doi: 10.1159/000449101

Figure Lengend Snippet: HMPV induced the expression of ER stress and unfolded protein response markers in NECs from both asthmatic and non-asthmatic subjects. Grp78 (a), sXBP1 (b), CHOP (c), GADD34 (d), PUMA (e) and NOXA (f) mRNA expression in NECs at 8, 12 and 24 h following infection with hMPV or RSV (Grp78 and sXBP1 only; MOI of 3). RT-qPCR was performed using total cellular RNA. Fold induction was calculated using 2−ΔΔCt normalized to the expression of β-actin gene. n = 5 for both asthmatic and non-asthmatic subjects. The bottom and top of the box plots represent the 5th and 95th percentiles, the bar represents the median and the whiskers represent minimum and maximum. A GEE approach was used to compare groups and the interaction between disease status and time (hMPV time course) or between disease status and virus (hMPV vs. RSV at 24 h p.i.) was not significant. * p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001 indicate statistical significance.

Article Snippet: RSV and hMPV intracellular RNA was quantified using SYBR green dye (QIAGEN), specific primers (online suppl. table S1 ; for all online suppl. material, see www.karger.com/doi/10.1159/000449101 ) and standard curves were generated using 10-fold serial dilutions of expression plasmids encoding the nucleocapsid (N) gene of RSV or hMPV (Biomatik).

Techniques: Expressing, Infection, Quantitative RT-PCR